Potential of high-performance liquid chromatography with photodiode array detection in forensic toxicology
Identifieur interne : 002872 ( Main/Exploration ); précédent : 002871; suivant : 002873Potential of high-performance liquid chromatography with photodiode array detection in forensic toxicology
Auteurs : Willy E. Lambert [Belgique] ; Jan F. Van Bocxlaer [Belgique] ; Andrép. De Leenheer [Belgique]Source :
- Journal of Chromatography B: Biomedical Sciences and Applications [ 0378-4347 ] ; 1997.
English descriptors
- Teeft :
- Acetonitrile, Adsorption chromatography, Alkaline conditions, Amine modifiers, Ammonium acetate, Ammonium nitrate, Anal, Array detection, Azide, Basic drugs, Blood sample, Bogusz, Capacity factors, Chloroquine, Chromatogr, Chromatographic, Chromatographic conditions, Cocaine, Crystalline residue, Dothiepin, Dothiepin sulphoxide, Eluent, Eluent composition, Forensic toxicology, Gradient elution, Gradient mode, High number, Hplc, Lambert, Liquid chromatography, Mass spectrometers, Mass spectrometry, Methanol, Mobile phase, Peak deconvolution, Peak identification, Photodiode array detection, Polybutadiene coating, Polydrug abuse, Postmortem specimens, Preston industries, Preston publications, Primary retention parameters, Real cases, Relevant drugs, Retention behaviour, Retention indexes, Retention times, Same brand, Same procedure, Silica, Single laboratory, Spectral data, Spectral information, Stomach contents, Systematic toxicological analysis, Toxic compounds, Toxicol, Toxicological, Toxicological analysis, Trazodone, Tricyclic antidepressants, Underivatized silica, Upper right, Urine, Urine sample.
Abstract
Abstract: The potentials and limitations of high-performance liquid chromatography-photodiode array detection are highlighted in respect to its use in the analysis of different biological matrices followed by the identification of unknowns. The logical analytical approach used in clinical and forensic toxicology, vital for the identification of one or more toxic substances as a cause of intoxication, is largely based on both simple and fast “general unknown screening” methods which cover most relevant drugs and potentially hazardous chemicals. In this field of systematic toxicological analysis, a literature overview shows that HPLC can play a substantial role. Both column packing material and eluent composition have their impact on intra- and interlaboratory reproducibility. In view of the sometimes different retention characteristics of various HPLC columns, several possibilities are addressed to enhance the discriminating power of primary retention parameters. The advantages of photodiode array detection as compared to UV detection have been of paramount importance to the success of HPLC in toxicological analysis. Dedicated libraries with spectral information and searching software are powerful tools in the process of identification of an unknown substance. In the present paper, these aspects are also verified in a number of real cases, i.e., trazodone and dothiepin, azide, chloroquine and cocaine, in which we illustrate from our own experience the potentials of HPLC-photodiode array detection in systematic toxicological analysis.
Url:
DOI: 10.1016/S0378-4347(96)00305-2
Affiliations:
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The logical analytical approach used in clinical and forensic toxicology, vital for the identification of one or more toxic substances as a cause of intoxication, is largely based on both simple and fast “general unknown screening” methods which cover most relevant drugs and potentially hazardous chemicals. In this field of systematic toxicological analysis, a literature overview shows that HPLC can play a substantial role. Both column packing material and eluent composition have their impact on intra- and interlaboratory reproducibility. In view of the sometimes different retention characteristics of various HPLC columns, several possibilities are addressed to enhance the discriminating power of primary retention parameters. The advantages of photodiode array detection as compared to UV detection have been of paramount importance to the success of HPLC in toxicological analysis. Dedicated libraries with spectral information and searching software are powerful tools in the process of identification of an unknown substance. In the present paper, these aspects are also verified in a number of real cases, i.e., trazodone and dothiepin, azide, chloroquine and cocaine, in which we illustrate from our own experience the potentials of HPLC-photodiode array detection in systematic toxicological analysis.</div></front></TEI><affiliations><list><country><li>Belgique</li></country><region><li>Province de Flandre-Orientale</li><li>Région flamande</li></region><settlement><li>Gand</li></settlement><orgName><li>Université de Gand</li></orgName></list><tree><country name="Belgique"><region name="Région flamande"><name sortKey="Lambert, Willy E" sort="Lambert, Willy E" uniqKey="Lambert W" first="Willy E." last="Lambert">Willy E. Lambert</name></region><name sortKey="De Leenheer, Andrep" sort="De Leenheer, Andrep" uniqKey="De Leenheer A" first="Andrép." last="De Leenheer">Andrép. De Leenheer</name><name sortKey="Van Bocxlaer, Jan F" sort="Van Bocxlaer, Jan F" uniqKey="Van Bocxlaer J" first="Jan F." last="Van Bocxlaer">Jan F. Van Bocxlaer</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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